A teenage male had a history of colitis, most likely Crohn’s disease. He had dif

Question

A teenage male had a history of colitis, most likely Crohn’s disease. He had difficulty controlling his disease with medical management and had been treated with parenteral nutrition and pain medication. He attends high school and is socially adjusted, even though his illness has caused him to be small in stature. He lives with his mother, who works for a veterinarian. The reason for this admission was abdominal discomfort and erythema at the exit site and along the tunnel of his central line. Blood cultures were collected, and both the blood cultures and his catheter tip cultures grew a catalase-positive, gram-positive cocci. The coagulase tube test was positive, but the slide and latex test for coagulase were negative

QUESTIONS

What further biochemical testing would you perform?

Because of the difficulties in expression of the mecA gene product in staphylococci, studies have been done to determine which antimicrobial agent best induces the microorganism to produce PBP2a. After extensive studies with many challenge strains, which antimicrobial agent was found to best predict the susceptibility or resistance to the penicillinase resistant penicillins?

Explanation / Answer

catalase-positive, gram-positive cocci are usually Staphylococcus or Microccous.

So the probability of Staphylococcus is high as it is known to cause infections at abdominal and gastrointestinal site. Mofeover her mom works for veterinarian there is a high possiblity that this strain has been transmitted from her to him. So the further test can be done to confirm is

1) atmospheric requirements,

(2) resistance to 0.04 U of bacitracin (Taxo A disk)

(3) resistance to 100-µg furazolidone, and

(4) possession of cytochrome C

Microdase disks, a modifed oxidase test, are used to differentiate Micrococcus spp. from Staphylococcus spp.and the probability that it could be MRSA.TO detect MRSA, CHROMagar could be used as it consist of an cefotixin to which MRSA is resistant to it.

2.  -lactam antibiotics induces the microorganism to produce PBP2a and can also help in predicting the resistance to the penicillinase resistant penicillins

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.