1) A mixture of A and B was partially separated on an HPLC column, resultin in o
ID: 783649 • Letter: 1
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1) A mixture of A and B was partially separated on an HPLC column, resultin in overlap of their chromatographic peaks. The absorbance of the effluent was monitored at 300nm (?A = 1800, ?B= 1600) and 350 nm (?A2100 and ?B=3200). The peak absorbance of the first chromatographic peak at 300nm was 0.020 and at 350nm 0.030. What is the main component of the first eluting peak and what percentage of the total absorbance at 300nm is due to this component?(assuming the light path- length = 1.0 cm) 1) A mixture of A and B was partially separated on an HPLC column, resultin in overlap of their chromatographic peaks. The absorbance of the effluent was monitored at 300nm (?A = 1800, ?B= 1600) and 350 nm (?A2100 and ?B=3200). The peak absorbance of the first chromatographic peak at 300nm was 0.020 and at 350nm 0.030. What is the main component of the first eluting peak and what percentage of the total absorbance at 300nm is due to this component?(assuming the light path- length = 1.0 cm)Explanation / Answer
use the 2nd principle and u'll get to the answer
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