PCR Amplification, sequencing and Genetic identification of Prokaryotes 1. Compa
ID: 77880 • Letter: P
Question
PCR Amplification, sequencing and Genetic identification of Prokaryotes
1. Compare DNA replication in a typical cell to the synthetic DNA replication that happens during the Polymerase Chain Reaction (PCR). Fill in the missing information that describes how each process is accomplished (i.e. enzyme name or other method) in each. Two answers are given as example:
2. Heating proteins to 94°C would destroy the function of most human or bacterial enzymes, including DNA Polymerase. How is this avoided during the DNA strand synthesis step in PCR?
3. What are the two major factors that influence the rate at which DNA amplicons move through an electrophoresis gel?
4. What are the three most significant features of the 16S rRNA gene that make it an excellent choice for DNA homology analysis and identification in bacteria?
Event Cell PCR Unwinding of DNA Strands DNA Helicase Primer Synthesis Synthetic primers added New DNA Strand synthesisExplanation / Answer
1.
Event
One strand synthesis is continuous other is discontinous ( semidiscontinuous synthesis)
2. Highly thermostable Enzyme, Taq DNA polymerase is used in PCR. This is not destroyed at 94°C. This enxyme is isolated from Thermus aquaticus, an extremophile found in hot water springs.
3. Two major factors are: size and configuration of DNA amplicon.
Larger molecules move slower than smaller ones.
Order of migration velocity of DNA molecules on the basis of configuration: supercoiled DNA> linear double stranded DNA> open circular DNA
4. Significant features of 16s rRNA gene:
Event
cell PCR Unwinding of DNA strands DNA helicase denaturation temperature, 94°C Primer synthesis primase synthesize RNA primers Synthetic primers added New DNA strand synthesisOne strand synthesis is continuous other is discontinous ( semidiscontinuous synthesis)
Continuous synthesisRelated Questions
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