PCR (polymerase chain reaction) allows target segments of DNA to be produced qui

Question

PCR (polymerase chain reaction) allows target segments of DNA to be produced quickly by using heat to denature and separate strands of DNA, then cooling to allow primers to attach to the single strands and DNA polymerase to synthesize new strands of the target DNA by adding nucleotides to the 3’ end of each primer on the two strands. Why is this process considered important?
a. It is useful for isolating the source of DNA.
b. It facilitates the introduction of a plasmid DNA into a host cell.
c. It enables selection of transformed cells that have taken up foreign DNA.
d. It can produce multiple copies of even minute samples of DNA.

Explanation / Answer

D-. It can produce multiple copies of even minute samples of DNA.

PCR cycles involved in three steps- denaturation,annealing and elongation.During annealing process DNA sample is cooled to 54 degree Celsius allowing primer ls attached to opposite ends of the target sequence.

A standard reaction of 30 cycles would yeald 1073741826 copies of DNA.

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