Answer ALL questions in this Section. 1. Which of the following is NOT a charact

Question

Answer ALL questions in this Section. 1. Which of the following is NOT a characteristic of prokaryoti single stranded DNA. c binding proteins? A. They are not essential for cell replication. B. They stimulate homologous DNA polymerase. C. They aid helicase action. D. They keep DNA strandsfrom annealing. E. They bind more tightly to ssDNA than to disDNA. 2. ADNA fragment was treated with EcoRIrestriction enzyme.The restriction pattern is shown below. How many fragments should be visible on a Southern blot if radioactively labeled probe"1" was used? DNA agents probe 1 probe 2 A. 0 B. 1 C. 2 D. 3 E. 4 3. Which of the following is used to study the interaction of proteins with DNA? A. si nuclease protection B. DNase-1 footprinting C. DNA fingerprinting D. southern analysis E. northern blotting

Explanation / Answer

1. SSBP donot stimulate DNA polymerase. So option B is the answer

2 Option D is the answer - 3 fragments, are visible where probe has bound to the restriction fragments.

3. Option B is the answer. DNAse foot printing is used to identify specific site of binding of a protein to DNA. Southern analysis is for detecting any DNA. Norther blot is for RNA detection.

4. Option D . luciferase is the reporter gene.

5. Option C. where a promoter region is linked to an easily detectable reporter gene, to measure the regulatory potential of a DNA sequence.

7.Option C.Removal of phosphate groups from DNA fragment can prevent ligation.

8, Option D is correct.

11. Option D is the answer, where cAMP binds to CAP. this binds to activator region, upstream promoter and helps RNA pol to bind.

13. Option C. DNA bending protein binds to enhancer andallows interaction between activaor and transcription factor.

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