Which of the following describes the semidiscontinuous replication of DNA? (I wa

Question

Which of the following describes the semidiscontinuous replication of DNA? (I was thinking it's answer B, but I am not sure.) A.) The leading strand is synthesized in the 5' -> 3' direction first. Upon completion, the lagging strand is synthesized, in pieces, in the 3' -> 5' direction. B.) The leading strand is synthesized in the 5' -> 3' direction, while the lagging strand is synthesized, in pieces, in the 3' -> 5' direction. C.) The leading strand is synthesized in the 5' -> 3' direction first. Upon completion, the lagging strand is synthesized, in pieces, in the 5' -> 3' direction. D.) The leading strand is synthesized in the 5' -> 3' direction, while the lagging strand is synthesized, in pieces, in the 5' -> 3' direction.

Match each of the following: Primosome DnaB DnaA p E. coli DNA ligase DnaG 1. DNA replication is initiated by a complex of up to 30 subunits of this protein 2. Hexameric helicase important in unwinding duplex DNA 3. Primase NAD+ dependent enzyme that seals nicked DNA 5. Protein complex that synthesizes RNA primers

Explanation / Answer

D.) The leading strand is synthesized in the 5' -> 3' direction, while the lagging strand is synthesized, in pieces, in the 5' -> 3' direction.

The answer is B. The replication of DNA is called to be semi discontinuous because the leading strand is synthesised in the 5'-->3' direction whereas the lagging strand is synthesised simultaneously in the opposite direction I.e. 3'----> 5' direction, in the form of Okazaki fragments. The synthesis of Okazaki fragments is termed as semidiscontinous synthesis.

Primosome - 5 ; protein synthesising RNA primer

E.coli DNA ligase- 4 ; NAD+ dependent enzyme that seals nicked DNA

DnaG- 3 ; primase

DnaB - 2; hexameric helicase important in unwinding duplex DNA

DnaA p - 1; DNA replication is initiated by a complex of up to 30 subunits.

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