Which of the following describes a likely model for the insertion of a simple tr

Question

Which of the following describes a likely model for the insertion of a simple transposon?

A characteristic host sequence is cleaved to generate staggered ends, the transposon with inverted repeat sequences at each end inserts between the staggered ends, and the staggered ends are filled in and ligated to the transposon.

A characteristic host sequence is cleaved to generate blunt ends, the transposon with inverted repeat sequences at each end inserts between the staggered ends, and the blunt ends are ligated to the transposon.

A characteristic host sequence is cleaved to generate staggered ends, the transposon with direct repeat sequences at each end inserts between the staggered ends, and the staggered ends are filled in and ligated to the transposon.

Any host sequence is cleaved to generate staggered ends of characteristic length, the transposon with inverted repeat sequences at each end inserts between the staggered ends, and the staggered ends are filled in and ligated to the transposon.

A characteristic host sequence is cleaved to generate staggered ends, the transposon with inverted repeat sequences at each end inserts between the staggered ends, and the staggered ends are filled in and ligated to the transposon.

Explanation / Answer

Simple transposon is an insertion sequence that contains it's own coding transposase between the short inverted repeated sequences that flank it's gene coding region.

Simple transposition is also called cut and paste transposition because the element is cut out of its original site and pasted to the new one.

It requires many transposase enzymes which cleaves specific host sequence and produce staggered ends.

The transposon which has inverted repeat sequences inserts between the staggered ends of host sequence which then gets ligated to the transposon.

So option ist is correct.

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