Using your data provide the answers to uestions a, b,c and d. The absorbance of
ID: 7710 • Letter: U
Question
Using your data provide the answers to uestions a, b,c and d. The absorbance of sample "a" at 405 nm is 0.035. The absorbance of sample "b" at 405 nm is o.150. The absorbance of sample "c" at 405 nm is 0.425.
A) Using your Standard Curve, determine the micromole amount of product in each sample.
"a"=0.001micromole; "b"=0.06micromole; "c"= 0.018..From my curve..
B) Using your "pH" experiment data assign a posible pH that each of these samples could have been drawn from. Be sure to indicate whether you selected "E" or "C" tubes from the pH experiment.
from My data...
ph Exper Abs Control ABS ExpABS-ControlABS micromoles product/15min
3 0.288 0.047 0.241 0.01
4 0.545 0.050 0.495 0.02
5 0.685 0.047 0.638 0.026
6 0.467 0.055 0.412 0.016
7 0.171 0.056 0.115 0.004
?
c) Consider all the "E" and "C" absorbance values you collected. Would you predict that all three tubes had enzyme added? Why or why not? Support your answer by citing data.
D) Is it possible that sample "b" is a control tube from one of the experiments? Is it possible that sample "c" is a control tube from one of the experiments? Why or why not?
Explanation / Answer
As we know the amount of product in each sample we can assign these 3 tubes to a particular pH:
A - 0.001mol - pH is 7
B - 0.06mol - pH is 5
C - 0.018mol - pH is 6.
This is done by basing on the amount of product in the data given.
We can determine whether the three tubes are E or C by basing on the abosrbance values:
A - 0.035 - aborbance control
B - 0.150 - Experimental absorbance
C- 0.425 - Experimental absorbance
only the B and C tubes had enzyme added as their absorbance values were quite higher than that of the A tube
B and C cannot be control tubes as their absorbance values are far higher than the values for the control tubes.
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