Dr. Rhoda Letter immunized a mouse with purified CD28, prepared splenocytes. fus

Question

Dr. Rhoda Letter immunized a mouse with purified CD28, prepared splenocytes. fused them with myeloma cells and isolated a hybridoma that made a monoclonal antibody specific for CD28. Dr. Letter purified a quantity of the monoclonal antibody. She wanted to prepare a polyclonal anti-idiotypic antibody to her anti-CD28 monoclonal antibody. To do that, she should immunize which of the following with the monoclonal antibody? a mouse genetically identical to the mouse from which she used to prepare the original monoclonal antibody; an allogeneic mouse; a rabbit; a syngeneic mouse except for the MHC-region. EXPLAIN

Explanation / Answer

Ans. a). a mouse genetically identical to the mouse from which she used to prepare the origional monoclonal antibody

Explan:-

The monoclonal antibodies used in the treatment first described are created by a procedure using mice. The first step in creating a monoclonal antibody is to immunize the mouse by giving it successive injections of the purest form of the addictive drug. Once the mouse is sufficiently immunized it is killed so that its spleen may be removed and harvested of its lymphocytes. After the lymphocytes are sufficiently separated and cleaned, they are mixed with myeloma. Since myeloma cells are able to replicate indefinitely, the fusion between the myeloma cells and the lymphocytes will allow the lymphocytes to replicate themselves. The remaining unfused cells are destroyed leaving only the hybridoma cells (hybrid lymphocyte-myeloma cells). The hybridoma cells are screened to find a few special cells that show the greatest potential for creating the desired antibody. These special hybridomas are separated and then encouraged to clone. Through this painstaking process of screening, cloning, and rescreening, hybridomas will be obtained that produce the wanted antibody. Once the correct hybridoma line has been obtained, an unlimited supply of supernatant containing the antibody can be collected. A higher concentration of the antibody can be produced by allowing the hybridomas to grow inside tumor cells within a mouse. Finally, complete monoclonal antibodies have been made for the specific addictive drug chosen.

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