In mapping a large plasmid you notice that EcoRI yields 3 bands, HindIII yields

Question

In mapping a large plasmid you notice that EcoRI yields 3 bands, HindIII yields four bands and you can detect at least 6 bands on the gel for HindIII/EcoRI digestion. Give answers to the following questions:

A. What is the inconsistency of these data?
B. What are two possible explanations?
C. How could you distinguish between these possibilities from the observation of the gel electrophoresis results?
D. How would your answers to the above questions change if you had obtained the same data from digesting a bacteriophage lambda clone?

Explanation / Answer

Number of bands is incorrent, should be 7, see 6

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