For each of the genotypes given below, predict whether active beta-galactosidase

Question

For each of the genotypes given below, predict whether active beta-galactosidase will be produced under two conditions: when the cell is not exposed to lactose and when it is exposed to lactose. Then predict whether active permease will be produced under the same two conditions. You can assume that there is no glucose in the system and any mutations in the Z gene are missense mutations. (a) I- P+ O+ Z+ Y+ (d) Is P+ O+ Z+ Y- / I+ P+ O+ Z- Y+ (b) Is P- Oc Z+ Y+ (e) I- P+ Oc Z- Y+ / I+ P+ O+ Z+ Y+ (c) Is P+ O+ Z+ Y- / I- P+ Oc Z- Y+ (f) I+ P+ O+ Z+ Y+ / Is P+ Oc Z+ Y-

Explanation / Answer

When lactose is not present When lactose is present a. I- P+ O+ Z+ Y+ Permease will be produced Permease will be produced b. Is P- Oc Z+ Y+ Permease will be produced Permease will be produced c. Is P+ O+ Z+ Y- / I- P+ Oc Z- Y+ Permease will be produced Permease will be produced d. Is P+ O+ Z+ Y- / I+ P+ O+ Z- Y+ Permease will not be produced Permease will not be produced e. I- P+ Oc Z- Y+ / I+ P+ O+ Z+ Y+ Permease will be produced Permease will be produced f. I+ P+ O+ Z+ Y+ / Is P+ Oc Z+ Y- Permease will not be produced permease will not be produced

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