Samples of a plasmid containing a segment of unknown DNA are digested using the

Question

Samples of a plasmid containing a segment of unknown DNA are digested using the restriction enzymes EcoRI, BamHI, and a combination of EcoRI and BamHI. The digests are then run on an agarose gel in order to separate the resulting fragments by size. Use the results of the gel electrophoresis (shown at right) to determine the sizes of the fragments and label the restriction map. Create a map of the DNA segment by dragging the enzyme name to the location it cuts (the vertical lines above the segment) and labeling the fragments with their lengths (numbers, in kb). You will not use all the numbers.

Explanation / Answer

Top of strand: BamHI, EcoRI, BamHI. Bottom of strand: 1kb, 4kb, 2kb, 3kb.

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