Many of the noncovalent interactions involving DNA are of this type? (a) Dipole

Question

Many of the noncovalent interactions involving DNA are of this type? (a) Dipole dipole or (b) Ionic

An antibiotic which prevents aminoacyl tRNA binding to small ribosomal (30S) ribosomal subunit in prokaryotes to inhibit bacterial protein synthesis (a) puromycin (b) tetracycline (c) erythromycin

An antibiotic which binds to the large (50S) ribosomal subunit in prokaryotes to inhibit bacterial protein synthesis: (a) puromycin (b) tetracycline (c) erythromycin

The Tm for melting the double helix is: (a) temp at which helix starts to open (b) midpoint of the range over which helix denatures (c) temperature at which helix is completely open (d) energy needed to melt the DNA (e) none of these

Explanation / Answer

Many of the noncovalent interactions involving DNA are of this type? (a) Dipole dipole or (b) Ionic

Ans. Dipole dipole interaction is present in DNA. As there are hydrogen bonds between bases of DNA.A double hydrogen bonded to T and C triple bond with G.

As Hydrogen bonds are also a type of dipole-dipole interactions that occur between hydrogen and either nitrogen, fluorine, or oxygen.

2. An antibiotic which prevents aminoacyl tRNA binding to small ribosomal (30S) ribosomal subunit in prokaryotes to inhibit bacterial protein synthesis (a) puromycin (b) tetracycline (c) erythromycin

Ans. Tetracycline is correct option. It inhibit protein synthesis by preventing the attachment of aminoacyl-tRNA to the ribosomal acceptor (A) site hence blocking this site after binding to 30s ribosomal subunit.

3.An antibiotic which binds to the large (50S) ribosomal subunit in prokaryotes to inhibit bacterial protein synthesis: (a) puromycin (b) tetracycline (c) erythromycin

Ans. Erythromycin is correct.

It inhibit bacterial protein synthesisby binding specifically to the 50s subunit of ribosome and impair the elongation cycle of the peptidyl chain.

4.The Tm for melting the double helix is: (a) temp at which helix starts to open (b) midpoint of the range over which helix denatures (c) temperature at which helix is completely open (d) energy needed to melt the DNA (e) none of these

Ans.(b) midpoint of the range over which helix denatures is correct.

because Tm is the temperature at which 50%of DNA helix exist as single strand and 50% intact duplex.

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