You are studying the biosynthesis of Compound X in a deep sea microorganism. You

Question

You are studying the biosynthesis of Compound X in a deep sea microorganism. You isolated five different strains of autotrophs that cannot synthesize Compound X on minimal media alone. Each mutant is tested for growth on minimal media plus the addition of a single compound in the biochemical pathway for Compound X. The results of that test are found below: Using these data, define the biosynthetic pathway (I put the pathway below, you just have to fill in the letter of the compound). Which step of the pathway is defective for each mutant? (Remember - over each arrow, we usually show the name of the enzyme that is involved at that step -i.e. enz A, enz B, etc)

Explanation / Answer

The order of Enzymes in the pathway are A - E - D- B- C - X

Strain Defective step of pathway/enzyme

Mutant 1 None

Mutant 2 Enzyme which converts B to C

Mutant 3 Enzyme which converts D to B

Mutant 4 Enzyme which converts E to D and A to E

Mutant 5 All the enzymes in the pathway

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