The below Figure Shows a Single DNA fluorescence histogram for cells of wild str

Question

The below Figure Shows a Single DNA fluorescence histogram for cells of wild strawberry plants (Fragaria vesca) and from commercially grown plants (Fragaria x ananassa) that have been mixed together. Circle the peak that represents fluorescence in cells of wild strawberry that are undergoing mitosis. Why do you think this peak represents this type of cell? Describe another method you could use to quantify differences in the amount of genomic material between wild and commercially grown strawberry calls. Genetics students collected data on their class to determine the frequency of the PTC-taster phenotype vs. the PTC-nontaster phenotype They found that 32 students were able to taste PTC and 10 students were unable to taste it Assuming that Hardy-Weinberg equilibrium applies, what is the frequency (p) of the PTC-taster allele in the overall population?

Explanation / Answer

In this experiment wild type strawberry (F. vesca) and commercially grown (f. ananassa) are used. If you look into the DNA content of both the species, we will find that wild type is 2n=2x=14 and commercially grown F. ananassa is octoploid with 2n= 8x= 56. Hence, F. ananassa has higher DNA content than wild type. Here in your question, the cells from both varieties are mixed together, and then fluorescence is measured. The cells are undergoing mitosis, which means the DNA is duplicating and hence there should be increase in fluorescence. So I feel that the third peak is the correct one.

You can quantify the difference in wild and commercial variety by observing absorption at 260nm i.e. spectrophotometry and determine the concentration of DNA.

2.

Hardy Weinberg equation

ptc taster frequency is 0.76

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.