Order the steps (1-6, 1 being the first step & 6 being the last step) of recombi

Question

Order the steps (1-6, 1 being the first step & 6 being the last step) of recombinant DNA formation, starting with isolation of the desired gene and bacterial plasmid and ending with mass production of the recombinant DNA.

1. Isolation of desired gene and bacterial plasmid

2. both the plasmid and the desired gene are cut using the same restriction enzyme

3. bacterial cells take up the recombinant DNA plasmid

4. DNA ligase is used to splice together the plasmid and the desired gene at their complementary sticky ends.

5. rapid reproduction of the bacterial cells produces many bacteria that contain the recombinant DNA.

6. Mass-production of recombinant DNA

Explanation / Answer

Following is the order of the steps:

1. Isolation of desired gene and bacterial plasmid

2. both the plasmid and the desired gene are cut using the same restriction enzyme

4. DNA ligase is used to splice together the plasmid and the desired gene at their complementary sticky ends.

3. bacterial cells take up the recombinant DNA plasmid

5. rapid reproduction of the bacterial cells produces many bacteria that contain the recombinant DNA.

6. Mass-production of recombinant DNA.

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