21. Which of the following is true regarding gel fi tration chromatography and S

Question

21. Which of the following is true regarding gel fi tration chromatography and SDS.PAGE? A) In both, small proteins move most rapidly. In PAGE, large proteins move most rapidly but in gel filtration, small proteins move most rapidly. D) B) C) In both, large proteins move most rapidly. In gel filtration, large proteins move most rapidly, but in PAGE, small proteins move most rapidly. E) None of the above. 22. You are interested in studying a powerful enzyme that is expressed in low amounts. Which of the following would you choose to determine how much is found in the tissue of interest? A) MALDI-TOF mass spectrometry to determine as much sequence data as you can. B) ELISA to identify any antigenic determinants. C) Amino Acid Composition Analysis because it can be done for the whole protein. D) 2D gel electrophoresis to determine charge and size data of the protein. E) Salting out to concentrate the protein for further study. 23. What determines a protein's function? A) N-terminal amino acids B) its gene sequence C) its structure D) None of the above. E) All of the above. Which of the following amino acid residues would most likely be buried in the interior of a water- soluble, globular protein? A) aspartate B) serine C) phenylalanine D) lysine E) glutamine 24.

Explanation / Answer

21-B

22-D

23-E (The function of a protein is determined by its shape. The shape of a protein is determined by its primary structure (sequence of amino acids). The sequence of amino acids in a protein is determined by the sequence of nucleotides in the gene (DNA) encoding it.)

24-C (phenylalanine)

( you are not asking for the explanation, so if you need the explanation then plz do comment in the comment section )

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