Suppose that you carry out an analytical procedure to generate a linear calibrat

Question

Suppose that you carry out an analytical procedure to generate a linear calibration curve like that shown in the figure below. Then you analyze an unknown and find an absorbance that gives a negative concentration for the analyte. What might this mean? (Select all true statements that apply.) 0.40 0.35 0.30 0.25 0.20 calibration 0.15 0.10 0.05 Unknown beyond linear region Unknown in linear region Linear calibration curve -0.05 0 10 15 20 25 Amount of protein (ug) It is possible to get values below O if you extrapolate the calibration curve past the range covered by standards, and the curve is not linear. If the negative value is within experimental error, no detectable analyte is present. If the negative concentration is beyond experimental error, there is something wrong with your analysis It is impossible to explain why you get values below 0 or above 100%

Explanation / Answer

Options 1st 2nd and 3rd are right choices.

Negative values can be ibtaiiob when the amount of analyte with respect to standard cakibration curve is low and negative values means that the values for analyte lies below the lower end of calibration curve.

Further more there is some experimental error if the value is within range of error it is the error that gives the negative values.

If it exceeds error range then either the analyte is impure or zero reading of experiment read some analyte conc that interfere with the conc of analyte.

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