You need to create 5 serial Dilutions of Glucose standards with a total volume o

Question

You need to create 5 serial Dilutions of Glucose standards with a total volume of 1 ml each. You are provided with a 300 mg/dL stock solution. The first concentration required 231 mg/dL is provided in the table below. Create a series of dilutions by cutting the concentration to 1/3 each time Complete the following table based on the use of the information above to make the following Molar concentrations of Glucose. (1 mL of total volume is used) (Give answers to 3 sigfigs): Volume (L) Glucose Needed (Serial Dilution) buffer (HL)Needed Volume Dilution Number [Glucose](mg/dL) [Glucose] (mol/L) Dilution 1 Dilution 2 Dilution 3 Dilution 4 Dilution 5 231

Explanation / Answer

Initial strength of glucose (S1)= 300mg/dL , Molar mass of glucose = 180g/mol

Final volume of each solution (V2) = 1mL

Formula used : V1S1=V2S2

1 L = 10 dL

Dilution no. [Glucose] (S2)(mg/dL) [Glucose](mol/L) Volume of Glucose (?L) [V1] Volume of buffer(?L) [1000-V1] 1 231 0.0128 770 230 2 77 4.278 x 10-3 256.7 743.3 3 25.67 1.426 x 10-3 85.6 914.4 4 8.555 4.75 x 10-4 28.5 971.5 5 2.852 1.58 x 10-4 9.506 990.494

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