You need to create 5 serial Dilutions of Creatinine standards with a total volum

Question

You need to create 5 serial Dilutions of Creatinine standards with a total volume of 1 mL each. You are provided with a 25 mg/dL stock Creatinine solution. The first concentration required is provided in the table below. Create a series of dilutions by cutting the concentration by 40% each time. Complete the following table based on the use of the information above to make the following PPM concentrations of Creatinine. (1 mL of total volume is used) (Give answers to 3 sigfigs):

Dilution # [Creatinine](mg/dL) [Creatinine](PPM) Volume (µL)
Creatinine Needed (Serial Dilution) Volume
Saline buffer (µL)Needed Dilution 1 24.5 245 980 20
   Dilution 2 14.7 147
  
  
Dilution 3 8.82 88.2
  
Dilution 4 5.29 52.9
  
  
Dilution 5 3.17 31.7
  
  

Explanation / Answer

Dilution creatinine mg/dL creatinine PPM Volume uL serial dilution volume saline buffer uL

1 24.5 245 980 20

2 14.7 147 588 12

3 8.82 88.2 352.8 7.2

4 5.29 52.9 211.68 4.32

5 3.17 31.7 127.008 2.592

CONVERSION OF mg/dL TO PPM ,

(24.5*10=245 ),

CUTTING Dillutions by 40% each,

1) 980*40=39200/100=392,

980-392=588, (980=100%, 392=40%),

20*40=800/100=8, (20-8=12 ), (20=100%, 8=40%),

2) 588*40=23520/100=235.5 (588-235.5=352.8 ),

12*40=480/100=4.8 (12-4.8=7.2 ,

3) 352.8*40%=14112/100=141.12 (352.8-141.12= 211.68,

7.2*40=288/100=2.88 (7.2-2.88=4.32 ),

4) 211.68*40=8467.2/100=84.67 (211.68-84.67= 127.008,

4.32*40=172.8/100=1.728 (4.32-1.728=2.592 .

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