The table below shows the data for purification of a hypothetical protein. Compl

Question

The table below shows the data for purification of a hypothetical protein. Complete the table by filling in th Map missing values for the specific activity, yield, and purification level Step Total Protein Total Activity Specific Activity Yield Purification (mg) 15100 4390 (units) 153000 138000 level (units mg-1) 10.1 31.4 (%) 100 90.1 Number Homogenization 3.11 Number 9.77 Salt fractionation Number lon-exchange chromatography 1410 113000 80.12 71.97 Number Number Number Gel-filtration chromatography 63.6 8.00 x 104 1257.8 50.95 153.39 Number Number Number Affinity chromatography 3.79 47900 12638.530.5 1541.28 Note: A red X will appear in the upper left corner of any box that has an incorrect answer f a box does not have a red X at the upper left, that particular answer is correct

Explanation / Answer

Formula: Specific Activity=Total Activity/Total Protein

Yield(%)=Total Activity of that step/Initial activity*100

Purification level=Specific Activity of that step/Initial Specific Activity

Step 3: For Ion-Exchange Chromatography, Total protein=1410 mg and total activity=113000.

Specific Activity=113000/1410 mg=80.1418 mg-1=80.14 mg-1

Yield=113000/153000*100=73.85 %

Purification level=80.14/10.1=7.934=7.93

Step 4: For Gel filtration Chromatography, Total protein=63.6 mg and total activity=8*104

Specific Activity=8*104/63.6 mg= 1257.86 mg-1

Yield=8*104/153000*100=52.287%=52.3%

Purification level=1257.8/10.1=124.53

Step 5: For Affinity chromatography, total protein=3.79 mg and total activity=47900

Specific activity=47900/3.79 mg=12638.5 mg-1

Yield(%)=47900/153000*100=31.30%

Purification level=12638.5/10.1=1251.33

Step Total Protein (mg) Total Activity Specific Activity Yield(%) Purification Level Homogenization 15100 153000 10.1 100 1 Salt fractionation 4390 138000 31.4 90.1 3.11 Ion-Exchange Chromatography 1410 113000 80.14 73.85 7.93 Gel filtration Chromatography 63.6 8*104 1257.8 52.3 124.53 Affinity Chromatography 3.79 47900 12638.5 31.3 1251.33

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