The table below shows the data for purification of a hypothetical protein. Compl

Question

The table below shows the data for purification of a hypothetical protein. Complete the table by filling in the missing values for the specific activity, yield, and purification level Step Total Protein Total Activity Specific Activity Yield Purification level (units) 139000 127000 (units mg-1) 11.1 35.2 (%) 100 91.1 Number (mg) Homogenization 12500 3.17 Salt fractionationn Number Number lon-exchange chromatography 108000 In 944 Number Number Number Gel-filtration chromatography 53.4 69400 Number Number Number Affinity chromatography 2.64 51300

Explanation / Answer

Specific Activity = Total enzyme activity / Total protein.

% Yield = Total enzyme activity / Starting total activity x 100.

Fold Purification = Specific activity / Initial specific activity.

Step Total protein Total activity Specific activity Yield(%) Purification level Homogenization 12500 139000 11.1 100 1 Salt fractionation 3610 127000 35.2 91.1 3.17 Ion-exchange chromatography 944 108000 114.4 77.7 10.3 Gel-filtration chromatography 53.4 69400 1299.6 49.9 117.1 Affinity chromatography 2.64 51300 19432 36.9 1750.6

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