2. Consider the mutations described in the left-hand column below. Identify whet
ID: 70231 • Letter: 2
Question
2. Consider the mutations described in the left-hand column below. Identify whether the Lac operon will be ON or OFF in each of the environments listed across the top.
Mutation
Glucose + Lactose
Lactose only
No Glucose or Lactose
Wild type
operon
OFF
ON
OFF
CAP site mutated
Operator mutated
CAP allosteric site mutated
Lac repressor allosteric site mutated
Lac repressor DNA binding domain mutated
Mutation
Glucose + Lactose
Lactose only
No Glucose or Lactose
Wild type
operon
OFF
ON
OFF
CAP site mutated
Operator mutated
CAP allosteric site mutated
Lac repressor allosteric site mutated
Lac repressor DNA binding domain mutated
Explanation / Answer
Mutation
Glucose + Lactose
Lactose only
No Glucose or Lactose
Wild type
operon
OFF
ON
OFF
CAP site mutated
Off
Off
Off
Operator mutated
Off
Off
Off
CAP allosteric site mutated
Off
Off
Off
Lac repressor allosteric site mutated
Off
Off
Off
Lac repressor DNA binding domain mutated
Off
On
Off
Mutation
Glucose + Lactose
Lactose only
No Glucose or Lactose
Wild type
operon
OFF
ON
OFF
CAP site mutated
Off
Off
Off
Operator mutated
Off
Off
Off
CAP allosteric site mutated
Off
Off
Off
Lac repressor allosteric site mutated
Off
Off
Off
Lac repressor DNA binding domain mutated
Off
On
Off
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