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Select items that describe a stage of a PCR reaction. Choose all that apply. DNA

ID: 70214 • Letter: S

Question

Select items that describe a stage of a PCR reaction. Choose all that apply.

DNA gyrase enzyme relieves the supercoiling ahead of the replication fork.

Primers bind to complementary sequences on the template DNA.

DNA is heated to a temperature that causes the strands to separate.

The DNA polymerase synthesizes a new strand of DNA that is complementary to the template DNA.

DNA gyrase enzyme relieves the supercoiling ahead of the replication fork.

Primers bind to complementary sequences on the template DNA.

DNA is heated to a temperature that causes the strands to separate.

The DNA polymerase synthesizes a new strand of DNA that is complementary to the template DNA.

Explanation / Answer

The polymerase chain reaction is the process used to amplify the single copy of DNA. This technique was developed by Kary Mullis. Thermal cycling is the method where repeated cycles are carried which includes repeated heating and cooling for DNA melting and enzymatic replication respectively. Primers bind to complementary target region along with DNA polymerase to synthesize new strand of DNA.

Thus, the stages of PCR reaction among the following are 2nd, 3rd, and 4th one.

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