A. Paper Chromatography 1. List below the solvent mixtures in 1-4 in order of in

Question

A. Paper Chromatography 1. List below the solvent mixtures in 1-4 in order of increasing polarity Mixture 1: 2 mL butanol 2 mL ethanol 2 mL ammonia Mixture 2: 1 mL butanol 2 mL ethanol 3 mL ammonia Mixture 3: 0 mL butanol 2 mL ethanol 4 mL ammonia Mixture 4: 4 mL butanol 2ml ethanol 0 mL ammonia Least Polar y Most Polar 2. In grammatically correct English, explain your reasoning for the above listing. we hive Clone adand w at th lution with nisre Aonia has mare rasing n the clor and the can Shown more clear l 6157 aA 3. Predict the effect of increasing solvent polarity on the Refactors of the spots 199

Explanation / Answer

1)Least polar

Mixture 4

Mixture 1

Mixture 2

Mixture 3

Most polar

2)Ammonia is most polar solvent and butanol is least polar solvent

Polarity of mixture increases on increasing ammonia and decreases on decreasing ammonia

Polarity decreases on increasing butanol

Ammonia is polar as it has large dipole moment

Butanol is ;east polar as it has large alkyl group

3) Rf=Distance moved by solute divided by distance moved by solvent

As polarity increases the solute moves to the top.So Rf value increases with increase in polarity

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