Suppose several unknown mutant strains of a bacterial species are tested for com

Question

Suppose several unknown mutant strains of a bacterial species are tested for complementary in with several known mutant strains of the same species. All of the known strains cannot synthesize proline and are designated pro-. Each known strain also has a different his3- mutation. On this bacterial species, the His3 protein contains multiple subunits. Indicated as A, B, and C, that each have a different catalytic function in the histidine biosynthesis pathway. The unknown bacterial strains are streaked across a minimal media playe that doesn't contain proline or histidine such that they will intersect and mate with the known strains. Control experiments, which are not shown, indicate no other deficiencies.
Mapd Suppose several unknown mutant strains of a bacterial species are tested for complementation with several known mutant strains of the same species. All of the known strains cannot synthesize proine and are designated pro". Each known strain also has a different his3" mutation. In this bacterial His3 protein contains multiple protein subunits, indicated as A, B, and C, that each have a different catalytic function in the histidine biosynthesis pathway. The unknown bacterial strains are streaked across a minimal media plate that does not contain proline or histidine such that they will intersect and mate with the known strains. Control experiments, which are not shown, indicate no othier deficiencies. the Use the information given in the table about the known strains and the results of the complementation test to determine the genotype of the unknown strains. Known Known strains genotype strainsgenotype genotype Unknown his3 pro his3a, pro4 his3b", pro his3c, pro6 2 his3b his3c his3 pro his3a

Explanation / Answer

1, 4 and 6 give positive complementation test, so they are different genes.

1 and 5 belong to same complementation group. So, genotype of 5 will be same as that of 1. His genotype will be his3a- and Pro genotype will be pro-

Similarly 2 and 6 belong to same complementation group. So, genotype of 2 and 6 will be same. So, genotype of 6 will be his3b- and pro-.

3 and 4 are same genes and belong to same complementation group. Genotype of 4 will be his3c- and pro-.

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