4. Enzyme rate dependence on temperature. 1.2 1.0 0.8 0.6 0.4 0.2 oL 60 80 100 4

Question

4. Enzyme rate dependence on temperature. 1.2 1.0 0.8 0.6 0.4 0.2 oL 60 80 100 40 Temperature, °C 20 The graph shown above is a plot of the rate of dopachrome formation versus temperature for the tyrosinase enzyme reaction. The substrate and enzyme concentrations were held constant. The only perimental parameter that was varied was the temperature. We know that as the temperature increases, the rate of a reaction should also increase. Explain in terms of the structure of the enzyme why the rate of the enzyme-catalyzed reaction initially increases, but then drops to zero at 95 ex

Explanation / Answer

Like most chemical reactions the rate of the reactions with increasing the temperature. But it rises to a certain temperature, reaches a maximum; here is at 600 C. But slowly it declines and it is due to above temperature 600 C, the enzyme starts denaturating and hence the rate of the reaction falls sharply. At 950C, complete denaturation of enzyme happens and hence the reaction drops at that temperature.  

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