You have isolated several different temperature sensitive mutant ycast strains t

Question

You have isolated several different temperature sensitive mutant ycast strains that stop proliferating when shifted from 25 degree C to 37 degree C. You analyze these mutant strains at the two different temperatures using FACS analysis in which a machine sons cells according to the amount of DNA they contain. At 25 degree C the FACS plots for all of the strains look like this: After the mutant cell cultures have been grown at 37 degree C you again analyze these cells using FACS. The plots below represent the different plot types from mutant yeast strains: For each of the following temperature sensitive mutations predict which FACS plot (A. B or C) you would expect (Circle the letter corresponding to the FACS plot you would expect) (1 point each) A B C An inactivating mutation in M-cyclin. A B C A mutation that prevented initiation of replication A B C A mutation that prevented the foflaospfrooiaiiflQ of S-Cdk ABC An inactivating mutation in the anaphase promoting comple

Explanation / Answer

An inactivating mutation in M-cyclin - A

A mutation that prevented initiation of replication – A

A mutation that prevented the dephosphorylation of S-Cdk – C

An inactivating mutation in the anaphase promoting complex (APC) – B

A mutation that stopped production of ATP - A

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