can someone help me with these question in genetic its for review for final exam

Question

can someone help me with these question in genetic its for review for final exam please try to answer all please and short answer iPad 1:29 PM 85% Genetics (BIOL-33060. 1. With diagrams, discuss the original DNA "Tingerprinting" technique as developed by Alec Jeffreys 2. Discuss how modern DNA profiling is performed using PCR, fluorescent nucleotides, capillary electrophoresis and STRs 3. Identifylexplain/discussletc. a. CODIS b. IrisPlex c. Innocence Project d. familial ONA search/profiling e. DBS1358 Home Page Tegrity Classes Presentations Videos and Animations Genetics Problems Syllabus Readings > g. SNP h. DNA Snapshoti. revised Cabridge Reference Sequence i-genomics k. Frederick Sanger lL GenBank 4. Why is population data important in forensic applications of DNA profling? 5. Discuss how DNA profiling based on physical characteristics can aid authorities in finding suspects. 6. What is the endosymbiont theory as developed by Lynn Margulis? What is the evidence in favor of it? 7. What gene products are encoded by human mtONA? 8. What are some human mtDNA diseases, and in what mtDNA do the mutations occur? 9 Discuss the procedure where a woman with an mtDNA disease can have a normal child using the ovum of a donor 10. How can mtDNA be used in profiling? 1. Why did the Department of Energy get involved with the Human Genome Project?

Explanation / Answer

2. Short tandem repeats (STR) are the shorter pieces of DNA (deoxyribonucleic acid), which would likely to be found in the areas of explosion, natural disasters and such situations where there are more chances of DNA destruction. Each person contains two copies of each STR. FBI (federal Bureau of Investigation) uses 13 STRs for DNA profiling Combines DNA Index System (CODIS) shares the DNA profiles among different crime laboratories.

Capillary electrophoresis is a technique used to isolate DNA fragments. Sample DNA is combined with fluorescently labelled short fragments of DNA that correspond to the parts of CODIS (Combined DNA Index System) STRs, this allows the complements to bind together. This is followed by PCR (polymerase chain reaction) that amplifies the genome (i.e. amplifying the all 13 CODIS markers simultaneously).

These DNA fragments are allowed pass through hair-thin tubes called “capillaries”. The fluorescent dye is now activated by applying a laser shone. Depend upon the number of amino acids preset in the fragment, the strength of fluorescence changes. For example for homozygous fragments, a single tall peak is obtained, and for heterozygous fragments two short peaks are obtained.

Every person inherits one genetic variant from the mother and other from the father. If the mother is known, we can match one variant with the mother genome and the other is checked with father’s genetic variants. If the genetic variants match then we can confirm the paternity. The child shares majority of the gene sequences with parents, this allows to track the parents and relatives. If around half of the CODIS sites match, the inference is that the people are considered first degree relatives i.e. may be father-son relation.

But similarly we can distinguish people because the polymorphs they inherit from their parents vary from person to person, for this to confirm a larger number of repeating units has to be tested.

Related Questions

Navigate

• Browse (All)
• Browse C
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.