can someone help me with these problems!?!? I am having a hard time figuring out

Question

can someone help me with these problems!?!? I am having a hard time figuring out the equations to use for them

Questions (10 pts): [show all calculations 1. You have a 32nt oligonucleotíde, calculate its approximate molecular weight (in 2. Calculate # grams needed to make 1mL of a 1M solution of the above 3. Take the IM solution, and make 1001 of a 250 solution. You cannot pipette 4. You have a 200M solution ofa primer (40nt) in a volume of 300L, how many 5. You received a primer (25nt) which came to you as a powder with a mass of Singrancle g/mol). oligonucleotide. less than 1uL, so you may need to do this in more than one step. Please show all calculations. dluHons ug of primer do you have total? 0.21mg. what concentration of primer do you have when you add 100L water? a. b. How would you make a 5mol/uL solution with a total volume of 100L.

Explanation / Answer

1. Molecular weight of Oligonucleotide = ( number of base pairs X average molecular weight of a deoxynucleotide monophosphate)

Number of base pairs= 32

average molecular weight of a deoxynucleotide monophosphate= 660 g/mol

So,   Molecular weight of Oligonucleotide = 32 X 660= 21120 g/mol

2. Moles ssDNA = mass of ssDNA (g)/molecular weight of ssDNA (g/mol)

1 = mass of ssDNA/ 21120

mass of ss DNA= 21120 gms

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