5. [2pt] In vitro translation systems have been developed in which specific RNA

Question

5. [2pt]
In vitro translation systems have been developed in which specific RNA molecules can be added to a test tube containing a bacterial cell extract that includes all the components needed for translation (ribosomes, tRNAs, amino acids). If a radioactively labeled amino acid is included, any protein translated from that RNA can be detected and displayed on a gel. If a eukaryotic mRNA is added to the test tube, would radioactive protein be produced? Explain.
Limit your answer to approximately 20 words.

6. [2pt]
In a comparable eukaryotic translation system (containing a cell extract from a eukaryotic cell), would a protein be produced by a polycistronic bacterial RNA? Would several proteins be produced? If not, why not?
Limit your answer to approximately 20 words.

Explanation / Answer

5. If it was an eukaryotic mRNA, it will not have the Shine dalgarno sequence present in bacterial mRNA. Since this interaction is absent, translation will not occur.

6. In eukaryotes, initiation factors are needed to kick start translation. These IFs depend on the 5' cap of mRNA.Since the mRNA from bacteria will not have the post-transcriptional modifications seen in eukaryotes, translation will not occur.

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