Question 2 (12 points) A purified DNA preparation of a certain plasmid is digest

Question

Question 2 (12 points)
A purified DNA preparation of a certain plasmid is digested to completion with BamH1 restriction endonuclease. In separate reactions, the same preparation was digested to completion with EcoRI and with a mixture of EcoRI and BamH1, respectively. The diagram below shows the appearance of the original molecules and the digestion products in the three digestion mixtures, after separation by electrophoresis in an agarose gel. (Note: Uncut supercoiled circular DNA migrates further in a gel because it is more compact in size than a linear DNA fragment of the same sequence length. The distance a supercoiled DNA molecule migrates on a gel is not an accurate indication of its sequence length.)

Lane M--Linear DNA Markers of the indicated length in kb
Lane 1--Sample of the original plasmid DNA preparation
Lane 2--Sample of the products of BamH1 digestion
Lane 3--Sample of the products of EcoRI digestion
Lane 4--Sample of the products of digestion of BamH1 + EcoRI

Answer and justify the following:
a. What was the original DNA molecule's length?
b. Was the original plasmid DNA circular or linear?
c. How many restriction sites for EcoRI and BamH1 did the original plasmid have?
d. Draw the original plasmid, using B and E to indicate the location of the BamH1 and the EcoRI restriction sites. Indicate the number of kb between sites.

Explanation / Answer

Dear sir please provide me the picture as i can not see the diagram here i am not able to answer this simple question. please attach diagram properly and send for answering again so i can have pleasure to help you out.

attache diagram separatly and not with the document.

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