Below are the compounds involved in glycolysis or citric acid cycle. At each com

Question

Below are the compounds involved in glycolysis or citric acid cycle. At each compound, the red color indicates the radioactive label, such as 14C label. These labeled compounds are useful for estimating the metabolic activity (glycolysis or citric acid cycle) by measuring the radioactivity in the resulting product. Determine what will be the fate of the radioactive label at the end of one citric acid cycle after glycolysis. Assume that each of following compounds is added to a mixture containing all enzymes and cofactors.

**Draw the all intermediate compounds and indicate the position of radiolabel at individual compound, and determine where it will be positioned at the end of pathways.

Explanation / Answer

The fate of radioactive labels will be:

a) At the end of one citric acid cycle label will emerge in C-2 and C-3 of oxaloacetate.

b) At the end of one citric acid cycle label will emerge in CO2. This CO2 will be in the formation of acetyl CoA from pyruvate.

c) At the end of one citric acid cycle label will emerge in C-1 and C-4 of oxaloacetate.

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