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http://www.ncbi.nlm.nih.gov/nuccore/NG_008185.1 A large biotechnology has subcon

ID: 67710 • Letter: H

Question

http://www.ncbi.nlm.nih.gov/nuccore/NG_008185.1

A large biotechnology has subcontracted your services to produce human lactate dehydrogenase. You decide to use DNA extracted from human fibroblasts to insert the gene into a bacterial plasmid. The lactate dehydrogenase gene has a length of 20417 base pairs. There are several introns in the gene. The exons are as follows (from http://www.ncbi.nlm.nih.gov/nuccore/NG_008185.1): Base pairs that form exons: 4955 5202 7385 7534 9997 10114 11403 11576 13406 13579 14260 14377 16015 16138 17683 18784 Moreover, on this sequence, the open reading frame spans from the position 7409 to 17847 on the DNA. What is the length of the messenger RNA (excluding the poly(A) tail)? What is the length of the polypeptide chain (number of amino acids prior to any posttranslational modifications)?

Explanation / Answer

2203 length of m-RNA.

610 aminiacids