What are the detection limits of spread and pour plat methods, assuming that you

Question

What are the detection limits of spread and pour plat methods, assuming that you are plating .1 and 1ml, respectively, of the undiluted broth sample.


I think - but not sure- t has something to do with the orignal values of the trypic soy broth (TBS) cultures
ecoli was approx 10^9 cfu/ml
lactobacillus sake was approx 10^8 CFU/ML
micrococcus leuteus was approx 10^8cfu/ml

and the values i recieved in my cfu from the morphology of the colonies..... the numbers were fairly close so i guess it gives a good representation of the original count?

I just dont know how to answer this question..... please help :)

Explanation / Answer

Detection limit is the lowest quantity of a substance that can be distinguished from the absence of that substance (a blank value) within a stated confidence limit. It can be calculated using the formula, Detection limit = student value * standard deviation Here given standard values are Ecoli was approx 10^9 cfu/ml Lactobacillus sake was approx 10^8 CFU/ML Micrococcus leuteus was approx 10^8cfu/ml Check out the number of CFU/ml in your culture plates and calculate the deviation and know the detection limit. As said, if the values received are close to the original values then the deviation would be minimum and detection limit will be equal to standard value. Hope it helps you.

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