3. (1 point) Consider the two samples of DNA shown below - single strands are sh

Question

3. (1 point) Consider the two samples of DNA shown below - single strands are shown for simplicity:

Sample #1
C A G T G A T C T C G A A TT C G C T A G T A A C G TT

Sample #2
T C A T G A A TT C C T G G A A T C A G C A A A T G C A

If both samples are treated with the restriction enzyme EcoRI [recognition sequence GAATTC, see introduction for details], indicate the number of fragments and the size of each fragment (consider the above single strands only) from each sample of DNA.

Sample # 1 Sample # 2

# of fragments:________ # of fragments:_________

List the sizes of DNA fragments following EcoRI digestion (length in number of bases) of both samples.

Explanation / Answer

sample#1: 2 fragments. 11bp and 17 bp

sample#2: 2 fragments. 5bp and 23bp. Both of these DNA fragments contain aisngle restrcition digestion site. Digestion will take place after G where this sign "^" has been shown. at 5'-G^AATTC-3'.

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