You are studying transcriptional regulation of a prokaryotic gene. You know this

Question

You are studying transcriptional regulation of a prokaryotic gene. You know this gene has one transcription regulatory protein that regulates its transcription, and there is one ligand molecule that allosterically affects the transcription regulatory protein. You do several experiments. In one experiment, you measure two things: whether the protein binds the DNA and whether mRNA is produced. You find that when the protein binds the DNA, mRNA of this gene is NOT produced. In another experiment, you measure binding of the ligand molecule to the protein and whether mRNA is produced. You find that when the ligand binds the protein, mRNA IS produced.

What kind of transcription regulator protein is this? (activator or repressor)

Is this an example of positive or negative regulation?

Does the ligand act as an inducer or inhibitor?

What level of structure in a transcription regulator protien determines the specific sequence of Dna it will bind to?

Explanation / Answer

What kind of the regulatory protein is this ?

ans; The regulatory protein is acting as a repressor

Is this an example of positive or negative regulation ?

ans; It is an example of the negative control

Does ligand act as an inducer or inhibhitor ?

ans ; ligand act as an inducer as binding to the regulatory protein will release the production of the m RNA

what level of the structure in a transcription regulator protein determines the specific sequence of the DNA it will bind to ?

They will have a DNA binding domain which will bind to the specific DNA sequences . DNA binding domain is a specific sequence in the DNA where the it contain atleast one motif that recognises the specific DNA sequences

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