You are studying several alleles of an E. coli helicase gene. One allele, called

Question

You are studying several alleles of an E. coli helicase gene. One allele, called rs, confers resistance to RS2014, an antibiotic that works by inhibiting helicase activity. Bacteria with the rsS allele are sensitive to RS2014. Another allele, called ts, produces a temperature sensitive mutation of the helicase. In bacteria with the ts' allele, helicase is inactivated at 42°C but functions normally at 37 °C, Helicase functions normally at both temperatureibcria wth the tat'alele. You obtain the following data while testing four strains of E. coli at different temperatures and doses of RS2014. Each number represents the percentage of maximal DNA synthesis. Based on this data, assign the appropriate genotype to strains A-D in the spaces provided. Percent of Maximal DNA synthesis Temperature (C) |37 |37 |37 |42 |42 |42 RS2014 dose (ug/m) 0 10 50 010 50 Strain Genotype ts 00436 100 41 7 1 100 100 100 100 100100 100 100 100 41 41 41 100 44 5 43 23 6 rss, ts

Explanation / Answer

Strain                   Genotype

A                          RSS, TS+

B                          RSR, TS+

C                          RSR, TS-

D                          RSS, TS-

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