Design the forward and reverse primers to mutate the following codons (just the

Question

Design the forward and reverse primers to mutate the following codons (just the internal primers, not the outside primers). Mutation primers are usually ~20 nucleotides long, only design them to be 9 nucleotides long to save time/work. Be sure to underline the mutant codon, and include at least 3 nucleotides from each side of the primer, so that your final primer is 9 nucleotides long. You must look up the genetic code.

15. 5’-TAAGGCTAGTAGACCGTATGCCGAATAC-3’

Mutate the threonine to cysteine

Internal forward: ___________________________ Internal reverse: ____________________________

Explanation / Answer

Forward primer: 5' - TAAGGCTAG - 3' or 5' - GTAGTGCGT - 3'

Reverse primer: 5' - ATACGCACT - 3' or 5' - GTATTCGG - 3'

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