Design mutations of hypothetical protein-coding genes: a) Design a null mutation

Question

Design mutations of hypothetical protein-coding genes: a) Design a null mutation where the site of the mutation is outside the Open Reading Frame (ORF) Explain how your mutation fits the definition of a null mutation [5 marks] 12 marks] c) Design a mutation that is not a null allele. Explain the consequence of your mutation for the b) What type of mutagen could generate your null mutation? Explain. function of the protein. [5 marks] d) What type of mutagen could generate your second mutation? Explain. 12 marks]

Explanation / Answer

Null allele is a non functional copy of the gene caused by a genetic mutation, this mutation can cause the completely lack of the production of the gene product or it can produce the non-functional gene product.

A) A mutation in pramoter sequence or in upstreem or downstreem ragulatory sequences which are away from the ORF sitll can cause a null allele. As we know these sequences are very important for the gene transcraption.

B) Any kid of a mutagen can cause a mutation in these reagins like Radiation based mutagens like X -ray, gamma rays, chemicals like EMS, MMS and others.

C) Conditional mutants. A mutant allele causes mutant phenotype in only a certian environment called the resractive environment but causes a wildtype environment in some different environment called permissive condation best example for this is temparature sensitive mutants.Here the mutation effects the protein folding i.e mutant protein folds normally at room temparature and follds abnormally and becomes non-functional at higher temparature.

D) One way to generate the temparature sensitive mutants is by using Diploid-shuffle method.

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