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Shown is a dsDNA sequence that a hypothetical human gene that codes for a very s

ID: 64708 • Letter: S

Question

Shown is a dsDNA sequence that a hypothetical human gene that codes for a very small protein. The Transcription Start Site (TSS) is shown after after the promoter (in yellow). Transcription proceeds in the direction of the arrow. Transcription stops at the end of the transcription termination site, shown in blue.

Which strand of the DNA, the top strand or the bottom strand, is the template strand?  

What is the sequence of the mRNA produced from this gene?

What is the sequence of the protein produced from the mRNA?

Suppose a mutation were found where a C/G (top/bottom) base pari were added immediately after the C/G base pair shown in purple.
What would be the sequence of the mRNA produced?
What would be the sequence of the protein produced?

Suppose a mutation were found where a T/A (top/bottom) base pari were added immediately after the T/A base pair shown in bold.
What would be the sequence of the mRNA produced?
What would be the sequence of the protein produced?

Explanation / Answer

B. mRNA sequences will be 5'GAGCCCAUGUUUCAGCAGGUCAUGUGAACUAAUAUCUC3'

poly peptide sequences will be EPMFQQVM.

C. mRNA sequences will be 5'GAGCCAUGUUUUCAGCAGGUCAUGUGAACUAAUAUCUC3'

poly peptide sequences will be EPCFQQVM

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