Pulse-chase experiments give organisms a brief pulse of a radiolabeled compound

Question

Pulse-chase experiments give organisms a brief pulse of a radiolabeled compound and then analyze the organism biochemically after a period of time to see where the radiolabel ends up (chase). You provide actively photosynthesizing plants C14 labeled CO2 for a period of 1hr and then return them to regular C12 CO2. You analyze the plants for radioactivity after 24 hours.

In which of the following tissues/organs will you find radiolabeled C14 (bold all that apply; 1pt)?

i.Leaves

ii.Stems

iii.Roots

iv.Flowers

v.Seeds

In which of the following molecules will you find the radiolabeled C14? (bold all that apply; 1pt)

i.Nucleotides/ nucleic acids

ii.Amino acids/proteins

iii.Carbohydrates/polysaccharides

iv.Lipids

Explain your answers to the previous 2 questions (2pts)

Explanation / Answer

In which organs the radiolabelled tissues/organs will you find the radiolabelled carbon in found ;

It will be present in the leaves as leaves are the organs where the photosynthesis process occurs .It will takes place in the stroma of the leaves .water enters the root and transport upto leaves through the specialized cells calledXylem vessels and the plants have specialized structures known as stomata to allow gas to enter and leave the leaf , carbondioxide can pass through stomata ,flanked by the two gaurd cells ,it occurs in a specialized cells called choloroplast .

In which of the following molecules you will find the radioactive labelled C14 is found ?

The C14 is present in the carbohydrates or polyssachrides , as the the end product of the photosynthesis is carbohydrates .The six molecules of the carbondioxide along with the water molecules will be converted into the carbohydrates .{mainly glucose]

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