2. The picture shown is the result of PCR amplification, restriction enzyme dige

Question

2. The picture shown is the result of PCR amplification, restriction enzyme digestion and agarose get electrophoresis of a DNA sample from a lab student. The first and second lanes are from DNA that was amplified but not treated with the restriction enzymes, Fnu4 and HaeIII, respectively. The second two lanes arc the same DNAs after treatment From looking at the before and after digestion, we can conclude that the DNAs did not cut with the enzymes. In our experiment, we expected that the taster people would have DNA that cuts with these enzymes, and the nontasters would not cut. a. From this information ONLY, what do you think the phenotype and genotype of this person (probably) are. Why is there a difference among these genotypes in whether their DNA cuts with these enzymes or not? b. It turns out that the person whose DNA is shown is actually a taster. How might you account for the discrepancy between the apparent phenotype and the genotype as determined by this DNA test? (There are several possible answers here).

Explanation / Answer

a. From the given statement, it is known that nontasters DNA would not have any cuts with Fnu4 and HaeIII. Therefore, observing the gel electrophoresis results and the given statement, we can say that the DNA is from nontasters.

b. If the DNA is from taster, then there might not be any restriction sites in that particular sequence of taster DNA. The proteins obtained from both the DNAs may vary resulting in different phenotypes.

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