How do we know that eukaryotic transcription factors bind to DNA sequences at or
ID: 63944 • Letter: H
Question
How do we know that eukaryotic transcription factors bind to DNA sequences at or near promoter regions?
A.
How do we know that double-stranded RNA molecules can control gene expression?
Chemical analysis, combined with optical (microscopic) and density analysis, determined that eukaryotic transcription factors bind to DNA sequences near promoter regions B. In experiments where transcription factors were labeled with 32P and promoters were labeled with 32S, autoradiography shown that these labels are located near each other. C. Transcription factors possess a DNA-binding domain that binds to DNA sequences and provides cis-regulation. Deletions of promoters and surrounding regions suppress binding of stranscription factorsExplanation / Answer
How do we know that promoter and enhancer sequences control the initiation of transcription in eukaryotes?
Mutations within promoters alter transcription efficiencies, whereas deletions alter the initiation point of transcription.
Enhancers are chromosomal elements that negatively influence transcription when deleted or altered by mutation. Insertion of an enhancer by recombinant technology increases transcription. Insertion of a silencer by recombinant technology decreased transcription.
How do we know that eukaryotic transcription factors bind to DNA sequences at or near promoter regions?
(A) Chemical analysis, combined with optical (microscopic) and density analysis, determined that eukaryotic transcription factors bind to DNA sequences near promoter regions
Explanation: As specific interactions between transcription factors and DNA responsive elements are important in understanding regulation of transcription, a number of methods like AFM and density analysis were used to identity the DNA sequence where transcription factors bind.
How do we know that double-stranded RNA molecules can control gene expression?
(A) After injection of certain short double-stranded RNAs in roundworm cells, specific mRNA degrades. This process is called RNA interference (RNAi).
Explanation : An exogenous dsRNA or endogenous pre-miRNA processed by dicer and can be incorporated in RNA-induced silencing complex (RISC), this will go and binds to the single-stranded messenger RNA molecules and causes translational repression, thus regulates the gene expression.
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