2. a) If there are 5 different VNTR alleles at the VNTR63 locus, then how many g
ID: 63632 • Letter: 2
Question
2.
a) If there are 5 different VNTR alleles at the VNTR63 locus, then how many genotypes would be possible in the population (ie V63-1/V63-2 would be one possible genotype)?
options:
a
5
b
10
c
15
d
20
e
25
f
70
b) If you perform PCR analysis of this region using the PL and PR primers, how many bands will you see on a gel from an individual with the following genotype:
V63-2/V63-2
options:
a
0
b
1
c
2
d
3
e
4
f
70
c) If you perform PCR analysis of this region using the PL and PR primers, how many bands will you see on a gel from an individual with the following genotype:
V63-4/V63-5
options:
a
0
b
1
c
2
d
3
e
4
f
70
d) If you perform PCR analysis of this region using the PL and PR primers, how many bands will you see on a gel from an individual with the following genotype:
V63-1/V63-2
options:
a
0
b
1
c
2
d
3
e
4
f
70
d) What will be the size of the DNA fragment in base pairs (bp) following amplification of V63-4 with the PL and PR primers as shown in the diagram on your worksheet (Q6)?
options:
a
10
b
30
c
40
d
50
e
70
f
80
e) The line below represents a mature eukaryotic mRNA. Place the following list of items in the proper order that you would find them on the mature mRNA.Note: some of the items may NOT be found on this mRNA.
5’--------------------------------------------------------------------------------------------3’
List of items:
Items to be ordered on the mRNA:
stop codon
poly-A tail
intron
3'-UTR
promoter
start codon
Kozak sequence
5'-cap
Shine-Delgarno Sequence
5'-UTR
Note: not all of the items may be on this particular mRNA
a
5
b
10
c
15
d
20
e
25
f
70
Explanation / Answer
1. (c) 15 ----- 1/1; 1/2; 1/3; 1/4; 1/5; 2/2; 2/3; 2/4; 2/5; 3/3; 3/4; 3/5; 4/4; 4/5; 5/5.
2. (b) 1 (homozygous genotype)
3. (c) 2 (heterozygous genotype)
4. (c) 2 (heterozygous genotype)
5. (e) 70 (15 nucleotides of P1, 15 nucleotides of P2, 10 nucleotides each of 1, 2, 3 and 4)
6. 5' CAP ----- 5' UTR ----- Shine-Delgarno sequence ----- Kozak Sequence ----- Start codon ----- Stop codon -----
3' UTR ----- Poly-A tail
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