6. Using figure 7.27 as your guide for VNTR analysis answer the following questi
ID: 63521 • Letter: 6
Question
6. Using figure 7.27 as your guide for VNTR analysis answer the following questions: Background: At locus VNTR63 there are 5 VNTR alleles (V63-1 to V63-5). Primers Left (Pi.) and Right (PR) are each 15-nts in length and are immediately adjacent to the VNTR repeats in the V63 region. Each VNTR63 repeat is 1O-bp in length. A person with VNTR63-2 has two VNTR63 repeats in the region while someone who has VNTR63-4 has four VNTR63 repeats in the region (as shown below in diagram). a. If there are 5 different VNTR alleles at the VNTR63 locus, then how many genotypes would be possible in the population (ie V63-1/V63-2 would be one genotype)? b. If you perform PCR analysis of this region using the P1 and PR primers, how many bands will you see on a gel from the following genotypes? 1. V63-2/V63-2 2. V63-4/V63-5 3. V63-1/V63-2 c. What will be the size of the DNA fragment in bp following PCR amplification of V63-4 with the PL and PR primers as shown in the diagram above?Explanation / Answer
a) nx (n+1)/ 2=5*6/2=15
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