The standard free energy deltaG0 for the alcohol dehydrogenase reaction is 5.5 k

Question

The standard free energy deltaG0 for the alcohol dehydrogenase reaction is 5.5 kcal/mole. Assume that you start with 1.5 x 10^-4 M ethanol and 8 x 10^-4 M NAD+, and run the alcohol deydrogenase reaction in the absence of semicarbazide. Formulas and constants to remember R = 1.98 cal/degree-mole ; Delta G0 = -RT lnKeq. T = 298 degrees K. A) Qualitatively, what will happen if the semicarbazide is left out? B) Quantitatively, how much NADH will be produced in the above reaction, assuming that no NADH or acetaldehyde was present initially?

Explanation / Answer

A) If semicarbazide is left out, an equilibrium is established for ADH and the acetaldehyde will, at some point, prevent further reaction.

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