3. In the experiment Chemical Composition of Cells you reached at the point in t

Question

3. In the experiment Chemical Composition of Cells you reached at the point in the experiment where you are dealing with coagulated extracted proteins. You add the solution of pancreatic enzymes and buffer to half your protein sample, and to the other half you add only buffer solution to serve as a control. While you then move on to another part of the experiment, one of your lab partners (who arrived home from an all - night dance at 4:00 that morning) hustles over to the test tubes that you have just finished working with and assumes that they contain glycogen. He adds 1N HCl to the tube that already contains the pancreatic enzymes and sets it in a boiling water bath. To the other tube he adds 1N HCI then immediately neutralizes with 1N NaOH. After 30 minutes, he discovers his error, doesn't tell anyone, removes the first test tube from the boiling water bath and places it, together with the second tube, in the basket marked ''protein incubation''. In the next day's lab, when your group's chromatogram develops, what results do the hydrolyzed protein and unhydrolyzed protein give, and why?

Explanation / Answer

The hydrolysed protein gives multiple spots on the chromatogram, while the hydrolysed protein sample gives only single spot. The difference is because, acid hydrolysis cleaves the protein into smaller peptides and amino acids, which results in the production of multiple spots.  

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