47. The universal primer described in class A. is complementary to a sequence in

Question

47. The universal primer described in class A. is complementary to a sequence in the plasmid that allows you to sequence a DNA inserted in the plasmid without prior knowledge of its sequence B. is complementary to a sequence in all known DNAs inserted in plasmids. C. is complementary to the conserved origin of DNA replication in the plasmid D. Is complementary to all RNAs transcribed from the E. coli genome. E. is a primer that is never useful for DNA sequencing. 48. Suppose a DNA in a genome has the following sequence: What primers would you use for PCR to amplify the sequence? A. 5'AAAAAAA3' and 5'CCCCCCC3 B. 5'AAAAAAA3 and 5'GGGGGGG3 C. 5'TTTTTTT3' and 5'ccCCCCC3 D. None of the above. 49. The most common current strategy for sequencing a genome involves A. partial digestion of the genome into overlapping DNA fragments. B. using a computer to detect the overlaps and order the DNA fragments and sequences within the genome. C. genetically mapping DNA fragments to determine their order before sequencing D. E. All of the above. Only A and B 50. Unequal recombination occurs between R1 and R4 on the two chromatids in the figure. This would generate two chromatids with A no Hb gene and two Hb genes B. no Hb gene and four Hb genes C. two Hb genes and four Hb genes. D. no Hb genes and six Hb genes. E. both chromatids would have three Hb genes. R1 R2 R3 R4 R1 R2 R3 R4 51. After transcription factor genes are duplicated by unequal recombination, over evolutionary periods of time, one transcription factor gene might A. move to a different chromosome. B. change the pattern of its gene transcription. C. change the target genes that it transcriptionally regulates D. All of the above.

Explanation / Answer

Universal primer is,

A. Complementary to a sequence in the plasmid that allows you to sequence a DNA inserted in the plasmid without prior knowledge of its sequence.

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