3. A company has created 3 types of tomatoes: red. pink and white. You want to u

Question

3. A company has created 3 types of tomatoes: red. pink and white. You want to understand why these tomatoes have different colors You know that the red gene contains a SNP in its promoter: The promoter sequence is shown below: the 33^rd nucleotide can be an A or a C. Because this SNP is in the TATA box of the red gene. you think that depending on whether this nucleotide is an A or a C. the gene will either be expressed (on = red allele) or remain silent (off= white allele). Based on this you generate a hypothesis that can possibly explain why tomatoes have different colors: if the red gene is off. the tomato will be white, if it is on it will be red and if only one allele is on. it will be pink. So you collect red, pink and white tomatoes and do several experiments to see if your hypothesis is true. The restriction endonucleases shown above recognize the following sequences: Experiment 1: You want to determine if the presence of the SNP at nt.#33 affects tomato color: to do this experiment, you obtain genomic DNA from 10 tomatoes that are red. pink and white tomatoes. You amplify the promoter region shown in the previous page for each tomato by PUR. To determine the presence of the SNP by PCR-RFLP you choose a restriction endonuclease from the list shown in the previous page. and perform PCR-RFLP. Your results are shown below. Assuming that your hypothesis is correct: based on these results which endonuclease could have been used in experiment la? Please explain your logic briefly.

Explanation / Answer

From the picture we can say there are two alleles. ssp1 have been used in this experiment which simply cuts palindromic sequences with much AT compared to GC..

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